I decided to move my stress promoter in front of an mCherry or red flourescent gene, and make a genetic construct that could also produce what looks like a red "ring of fire" (above). I am hopeful that what is observed at the bottom of this flask contains cells expressing red fluorescent proteins (RFP) under control of my genetic stress switch.
After the cloning, cells where grown overnight and centrifuged down to make a dense concentrate. I will verify that the construct is correct, but the strong red colour is at least a hopeful indicator.
Last year, I made a green fluorescent construct but RFP can be more difficult to express for bacteria. Despite this, once expressed, I think RFP is a more dramatic identifier.